LOEWE Center for
Insect Biotechnology
& Bioresources

Tanja Rehling

Technical assistant

Phone: +49 641 99-39517

Email: tanja.rehling@agrar.uni-giessen.de

Room: 2.43

Building: TIG

Vita

Since 01/2017 Technical assistant in the group of Dr. Marc F. Schetelig at the LOEWE-Center for Insect Biotechnology and Bioresources, Justus-Liebig-University Gießen, Germany
04/201412/2016 Technical assistant in the group of Dr. rer. nat. Marc F. Schetelig at the LOEWE-Centre for Insect Biotechnology and Bioresources
02/2011 – 04/2014 Technical assistant in the project SFB/Transregio 79 – „Materials for Tissue Regeneration in Systemically Diseased Bones“ in the Laboratory of Experimental Trauma Surgery, Justus-Liebig-University, Giessen
08/1987 – 02/2011 Technical assistant in the field of clinical and physiological research and studies at the Max-Planck-Gesellschaft, Clin. Res. Unit for Blood Coagulation, Giessen and Max-Planck-Institute for Clinical and Physiological Research, Bad Nauheim.

Research Interests

current project:

Since April 2014 in the group of Prof. Dr. Marc F. Schetelig. The group develops and evaluates transgenic insects, such as mosquitos and fruit flies, for use in insect pest control programs.
This work and responsibilities consist of Mosquito rearing, Mosquito germline transformation via embryonic microinjection, genetic crosses and screening for transgenics, molecular sequence analysis with Geneious Software, inverse PCR, expression analysis with qPCR, intetegration analysis, droplet digital PCR, creating cloning strategies and cloning of plasmid vectors.

professional experience:                       

From Feb. 2011 to April 2014 as technical assistant in the project SFB/Transregio 79 – „Materials for Tissue Regeneration in Systemically Diseased Bones“ in the Laboratory of Experimental Trauma Surgery, Justus-Liebig-University, Giessen. The focus of the work was on developing suitable animal models and testing newly developed biomaterials. This work included animal surgeries and euthanasia, collecting tissue and bone samples, sectioning,  histological, immunohistochemical and enzyme staining and their histomorphometric evaluation. Additionally development and validation of PCR and qPCR studies for gene expression analysis of markers of bone formation and resorption and evaluation of the results for the publications was a large part of the work.

From Aug.1987 to Feb. 2011 as technical assistant in the field of clinical and physiological research and studies at the Max-Planck-Gesellschaft, Clin. Res. Unit for Blood Coagulation, Giessen and Max-Planck-Institute for Clinical and Physiological Research, Bad Nauheim. This work involved cloning of plasmids, transformation, transfection, expression in cell lines and evaluation of the recombinant coagulation factors as well as purification and characterization of proteins from plasma, preparation of monoclonal and polyclonal antibodies, screening of patients for genetic defects associated with thrombophilia and clinical diagnosis in the central laboratory of the Kerckhoff Clinik.

Publications

Ramírez-Santos E, Rendón P, Bourtzis K, Schetelig MF, Cáceres C, Targovska A, Rehling T, Guillén K, Ruiz-Montoya L, Toledo J, Liedo P (2018) Generational interaction of a transgenic Ceratitis capitata (Tephritidae) strain and the parasitoid, Fopius ceratitivorus (Braconidae), as a model to assess the risk of horizontal gene transfer in eukaryotes. , under review
Thormann U, Ray S, Sommer U, El Khassawna T, Rehling T, Hundgeburth M, Henß A, Rohnke M, Janek J, Lips KS, Heiss C, Schlewitz G, Szalay G, Schumacher M, Gelinsky M, Schnettler R, Alt V (2013) Bone formation induced by strontium modified calcium phosphate cement in critical-size metaphyseal fracture defects in ovariectomized rats Biomaterials, 34 (34) , 8589–8598.