LOEWE Center for
Insect Biotechnology
& Bioresources

Denise Salzig

Dr.-Ing.

Group leader

Phone: +49 641 309-2634

Email: denise.salzig@kmub.thm.de

Room: D10.115

Building: THM

Vita

since 2013 Group leader LOEWE ZIB, THM, Gießen
since 2009 Head of the cell culture technology section and project leader after § 15 Abs 4 GenTSV, Institute of Bioprocess Enginnering and Pharmaceutical Technology, Prof. Czermak, THM, Gießen
2008–2009 Post-Doc, Institute of Bioprocess Enginnering and Pharmaceutical Technology, Prof. Czermak, THM, Gießen
2004-2008 PhD, Chair of bioprocess technology, Prof. Freitag, University of Bayreuth
1999-2004 diploma biochemistry, University of Greifswald, University of Bayreuth

Research Interests

  • recombinant peptide/protein expression in prokaryotes, yeast and insect cells
  • new recombinant expression systems for bioprocess development
  • alternative purification strategies for recombinant peptides
  • expansion processes of stem cells for cell therapy purposes
  • production processes of oncolytic viruses
  • bioprocess engineering, process analytical technology

Member of the European Society of Artifical Organs (ESAO)

Patents

EP2473594 (A2) ― 2012-07-11
CZERMAK PETER [DE]; FREIMARK DENISE [DE]; GRACE PABLO PINO [DE]; JUSTICE CHRISTIANE [DE]; WEBER CHRISTIAN [DE]
Device and method for the expansion, harvesting and differentiation of stem cells.


Publications

Grein TA, Loewe D, Dieken H, Salzig D, Weidner T, Czermak P (2018) High titer oncolytic measles virus production process by integration of dielectric spectroscopy as online monitoring system Biotechnology and Bioengineering, in press
Leber J, Barekzai J, Blumenstock M, Pospisil B, Salzig D, Czermak P (2017 (in press)) Microcarrier choice and bead-to-bead transfer for human mesenchymal stem cells in serum-containing and chemically defined media. Process Biochemistry, 59 (B) , 255-265.
Grein TA, Schwebel F, Kress M, Loewe D, Dieken H, Salzig D, Weidner T, Czermak P (2017) Screening different host cell lines for the dynamic production of measles virus Biotechnology Progress, 33 (4) , 989-997.
Sprick G, Weidner T, Salzig D, Czermak P (2017) Baculovirus-induced recombinant protein expression in human mesenchymal stromal stem cells: A promoter study. New Biotechnology, 39 (B) , 161-166.
Grein TA, Leber J, Blumenstock M, Petry F, Weidner T, Salzig D, Czermak P (2016) Multiphase mixing characteristics in a microcarrier-based stirred tank bioreactor suitable for human mesenchymal stem cell expansion. Process Biochemistry, 51 (9)
Köster N, Schmiermund A, Grubelnig S, Leber J, Ehlicke F, Czermak P, Salzig D (2016) Single-Step RNA Extraction from Different Hydrogel-Embedded Mesenchymal Stem Cells for Quantitative Reverse Transcription–Polymerase Chain Reaction Analysis. Tissue Engineering Part C: Methods, 22 (6) , 552–560.
Moghaddam MR, Tonk M, Schreiber C, Salzig D, Czermak P, Vilcinskas A, Rahnamaeian M (2016) The potential of the Galleria mellonella innate immune system is maximized by the co–presentation of diverse antimicrobial peptides. Biological Chemistry, epub ahead of print
Petry F, Smith JR, Leber J, Salzig D, Czermak P, Weiss ML (2016) Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use. Stem Cells International, 2016 (ID 4834616) , 1–12.
Salzig D, Leber J, Merkewitz K, Lange MC, Köster N, Czermak P (2016) Attachment, Growth, and Detachment of Human Mesenchymal Stem Cells in a Chemically Defined Medium. Stem Cells International, 2016 (ID 5246584) , 1–10.
Druzinec D, Salzig D, Kraume M, Czermak P (2015) Micro-bubble Aeration in Turbulent Stirred Bioreactors: Coalescence Behavior in Pluronic F68 Containing Cell Culture Media Chemical Engineering Science, 126 , 160–168.
Eisenhardt M, Dobler D, Schlupp P, Schmidts T, Salzig M, Vilcinskas A, Salzig D, Czermak P, Keusgen M, Runkel F (2015) Development of an insect metalloproteinase inhibitor drug carrier system for application in chronic wound infections Journal of Pharmacy and Pharmacology , 67 (11) , 1481-1491.
Elseberg CL, Salzig D, Czermak P (2015) Bioreactor expansion of human mesenchymal stem cells according to GMP requirements Stem Cells and Good Manufacturing Practices , 1283 in Methods in Molecular Biology, Turksen K (Eds.) , 199–218. Springer: Berlin Heidelberg
Kuhn J, Müller H, Salzig D, Czermak P (2015) A new method for a rapid glycerol determination during microbial fermentation Electronic Journal of Biotechnology, 18 (3) , 252–255.
Müller H, Salzig D, Czermak P (2015) Considerations for the process development of insect-derived antimicrobial peptide production Biotechnology Progress, 31 (1) , 1–11.
Weiss K, Gerstenberger J, Salzig D, Mühlebach MD, Cichutek K, Pörtner P, Czermak P (2015) Oncolytic measles viruses produced at different scales under serum-free conditions Engineering in Life Sciences, 15 (4) , 425–436.
Cierpka K, Mika N, Lange MC, Zorn H, Czermak P, Salzig D (2014) Cell detachment by prolyl-specific endopeptidase from Wolfiporia cocos American Journal of Biochemistry and Biotechnology, 10 (1) , 14–21.
Druzinec D, Weiss K, Elseberg C, Salzig D, Kraume M, Pörtner R, Czermak P (2014) Process analytical technology (PAT) in insect and mammalian cell culture processes: dielectric spectroscopy and focused beam reflectance measurement (FBRM) Methods in Molecular Biology, 1104 , 313–341.
Oppermann T,Leber J, Elseberg C, Salzig D, Czermak P (2014) hMSC production in disposable bioreactors in compliance with cGMP guidelines and PAT American Pharmaceutical Review,
Cierpka K, Elseberg CL, Knut N, Kassem M, Salzig D, Czermak P (2013) hMSC production in disposable bioreactors with regards to GMP and PAT Chemie Ingenieur Technik, 85 (1-2) , 67–75.
Druzinec D, Salzig D, Brix A, Kraume M, Vilcinskas A, Kollewe C, Czermak P (2013) Optimization of Insect Cell Based Protein Production Processes - Online Monitoring, Expression Systems, Scale-Up Advances in Biochemical Engineering/Biotechnology, 136 , 65–100.
Gebauer E, Goßla E, Kwas C, Salzig D, Schmiermund A, Czermak P, Fuchsbauer HL (2013) Identification of transglutaminase substrates from porcine nucleus pulposus as potential amplifiers in cross-linking cell scaffolds Biomacromolecules, 14 (5) , 1564–1571.
Salzig D, Schmiermund A, Grace PP, Elseberg C, Weber C, Czermak P (2013) Enzymatic detachment of therapeutic mesenchymal stromal cells grown on glass carriers in a bioreactor The Open Biomedical Engineering Journal, 7 , 147–158.
Weiss K, Salzig D, Röder Y, Gerstenberger J, Mühlebach MD, Cichutek K, Pörtner R, Czermak P (2013) Influence of process conditions on measles virus stability American Journal of Biochemistry and Biotechnology, 9 (3) , 243–254.
Elseberg CL, Justice C, Leber J, Salzig D, Wallrapp C, Kraume M, Kassem M, Czermak P (2012) Microcarrier-based expansion process for hMSCs with high vitality and undifferentiated characteristics. The International Journal of Artificial Organs, 35 (2) , 93–107.
Salzig D, Jérôme V, Freitag R (2012) Effect of process parameters and product-host-interaction on hVEGFA-production by recombinant Chinese hamster ovary cells Biotechnology Progress, 28 (3) , 762-772.
Weiss K, Salzig D, Mühlebach MD, Cichutek K, Pörtner R, Czermak P (2012) Key parameters of measles virus production for oncolytic virotherapy. American Journal of Biochemistry and Biotechnology, 8 (2) , 81-98.
Freimark D, Sehl C, Weber C, Hudel K, Czermak P, Hofmann N, Spindler R, Glasmacher B (2011) Systematic parameter optimization of a Me2SO- and serum-free cryopreservation protocol for human mesenchymal stem cells. Cryobiology, 63 (2) , 67–75.
Justice C, Brix A, Freimark D, Kraue M, Pfromm P, Eichenmueller B, Czermak P (2011) Process Control in Cell Culture Technology Using Impedance Spectroscopy. Biotechnology Advances, 29 (4) , 391–401.
Justice CL, Leber J, Freimark D, Pino Grace P, Kraume M, Czermak P (2011) Online- and offline- monitoring of stem cell expansion on microcarrier Cytotechnology, 63 (4) , 325–335.
Salzig D, Schmiermund A, Gebauer E, Fuchsbauer HL, Czermak P (2011) Influence of porcine intervertebral disc matrix on stem cell differentiation Journal of Functional Biomaterials 2, , 155-172.
Salzig D, Schmiermund A, Gebauer E, Fuchsbauer L, Czermak P (2011) Influence of porcine nucleus pulposus extract on differentiation of human mesenchymal stem cells for intervertebral disc regeneration Biomaterialien 12, , 197.
Ehlicke F, Freimark D, Heil B, Dorresteijn A, Czermak P (2010) Intervertebral Disc Regeneration: Influence of growth factors on differentiation of human mesenchymal stem cells (hMSC) Int J Artif Organs, , 244-252.
Freimark D, Grace PP, Weber C, Pohl S, Poertner R, Wallrapp C, Geigle P, Czermak P (2010) Use of encapsulated stem cells to overcome the bottleneck of cell availability for cell therapy approaches Transfusion Medicine and Hemotherapy 37, , 66-73.
Freimark D, Jérôme V, Freitag R (2010) A GFP-based method facilitates clonal selection of transfected CHO cells Biotechnology Journal, 5 (1) , 24-31.
Grein T, Freimark D, Weber C, Hudel K, Wallrapp C, Czermak P (2010) Alternatives to dimethylsulfoxide for serum-free cryopreservation of human mesenchymal stem cells Int J Artif Organs, , 370-380.
Weber C, Pohl S, Poertner R, Grace PP, Freimark D, Wallrapp C, Geigle P, Czermak P (2010) Expansion of human mesenchymal stem cells in a fixed-bed bioreactor system – Part B: Modeling and scale up Int J Artif Organs, , 782-795.
Weber C, Pohl S, Poertner R, Grace PP, Freimark D, Wallrapp C, Geigle P, Czermak P (2010) Expansion of human mesenchymal stem cells in a fixed-bed bioreactor system – Part A: Inoculation, cultivation, cell harvest procedures Int J Artif Organs, , 512-525.
Weber C, Pohl S, Poertner R, Grace PP, Freimark D, Wallrapp C, Geigle P, Czermak P (2010) Production process for stem cell-based therapeutic implants - Expansion of the production cell line and cultivation of encapsulated cells Advances in Biochemical Engineering/Biotechnology 123, , 143-162.
Freimark D (2008) Entwicklung und Charakterisierung von Bioprozessen zur Produktion osteoinduktiver Wachstumsfaktoren in chinese hamster ovary Zellen , , 1-256. Verlag Dr. Köster: Berlin