Seminar announcement 11. Jun 2014
Vassiliki Bariami (PhD)
P450s, ABC transporters and lipid metabolism genes: Unravelling the molecular basis of pyrethroid resistance in the dengue vector Aedes aegypti.
Pyrethroid insecticides are widely utilized in dengue control. However, the major vector, Aedes aegypti, is becoming increasingly resistant to these insecticides and this is impacting on the efficacy of control measures. The near complete transcriptome of three pyrethroid resistant populations from the Caribbean was examined to explore the molecular basis of this resistance. Approximately 32.5% per cent of the genes over-expressed in resistant mosquitoes were up-regulated in all Caribbean populations. Genes with monooxygenase activity (P450s), ABC transporters and lipid metabolism genes were significantly over represented in the up-regulated subset in all Caribbean populations. Two members of the CYP9 subfamily (CYP9J26 and CYP9J28) and two previously uncharacterized genes, classified in the lipid metabolism group (ApoD and LIPA), were chosen for further investigation. To functionally validate the roles of these genes in the establishment of resistance, heterologous overexpression in the model insect Drosophila melanogaster was employed.
Vassiliki Bariami (PhD), JLU Giessen